I compiled Linux-Vfio for Arch and patched the kernel.
Everything works fine now.

The GPU are in the correct slots, the IOMMU groups are separated, and now I can even remove the HDMI audio from the R9 270x GPU using the QEMU command.
In short, ACS is in the TUF GAMING BIOS, but unfortunately, it has some limitations, so it can't be used for AmigaOS emulation in this case.
I'm happy now that I can log in to Linux directly on the primary monitor without any strange issues.
And then I can use the R9 270x on the second monitor completely normally, without the problems I had before.
I don't have to plug and unplug HDMI cables all the time.

Well,
Unbelievable!
Between audio not working and ACS on the motherboard, it was a real obstacle course for me.
